Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: CircRNA_103827 and circRNA_104816 expressions in granulosa cells according to patients’ clinical characteristics.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Biomarker Discovery

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: CircRNA_103827 and circRNA_104816 expression levels in granulosa cells according to ART outcomes.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: The top 10 significantly differentially expressed circRNAs between young and older women ranked by fold change.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques:

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: Comparison of candidate circRNAs (circRNA_103829, circRNA_10827, circRNA_104816, circRNA_101889, circRNA_103828, circRNA_100833, circRNA_104852, circRNA_103611) expression levels in granulosa cells from additional young (n = 20) and older (n = 20) women by qRT-PCR. YA, women with young age (≤ 30 years); AA, women with advanced age (≥ 38 years). P values were calculated by Mann-Whitney U test. Relative expressions were analyzed by 2 -ΔΔCt method which was normalized to GAPDH. For each box plotting, the central mark represents the median, the edges of the box represent the 25 th and 75 th percentiles, and the whiskers are the most extreme data points not considered outliers.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Comparison, Expressing, Quantitative RT-PCR, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: ( A-E ) circRNA_103827 expression levels in granulosa cells according to (A) serum AMH levels, (B) AFC, (C) retrieved oocytes, (D) top quality embryos and (E) top quality embryo percentage. ( F-J ) circRNA_104816 expression levels in granulosa cells according to (F) serum AMH levels, (G) AFC, (H) retrieved oocytes, (I) top quality embryos and (J) top quality embryo percentage.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: ROC analysis to evaluate predictive performance of circRNA_103827, circRNA_104816 expressions in granulosa cells and top quality embryo proportion for ( A-C ) clinical pregnancy outcomes, as well as for ( D-F ) live births. AUC, area under the ROC curve.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques:

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: (A) CircRNA_103827/circRNA_104816 targeted “Top 5” miRNA-gene network was portrayed based on sequence-pairing prediction. A pink round node represents a gene, a blue square represents miRNA, and a yellow diamond represents a circRNA. Overlapping genes of both circRNAs in this interactive network were ANKRD20A9P , XIST and KCNQ1OT1 . (B) GO and (C) KEGG pathway analysis of circRNA_103827 and circRNA_104816 predicted target genes. The top 10 significantly enriched activities and their scores (negative logarithm of P value) were listed in the X-axis and the Y-axis, respectively.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Sequencing

Journal: Stroke

Article Title: Circular RNA expression Profiles Alter Significantly in Mouse Brain after Transient Focal Ischemia

doi: 10.1161/STROKEAHA.117.017469

Figure Lengend Snippet: Majority (~80%) of the circRNAs altered after stroke are exonic (A). The circRNAs altered after stroke were transcribed from all 20 chromosomes (B). Real-time PCR confirmed the microarray data for 6 circRNAs (3 upregulated and 3 downregulated) at 6h reperfusion compared to sham (C). Values are mean ± SD (n =3/group). Real-time PCR analysis was conducted in triplicate. *p<0.05 compared to sham (Student’s T-test).

Article Snippet: The fluorophore-labeled cRNAs were purified (RNeasy Mini Kit; Qiagen USA) and 1 μg sample of each labeled cRNA was fragmented (60°C for 30 min in fragmentation buffer/blocking buffer; Arraystar USA), suspended in hybridization buffer and hybridized to a mouse circRNA microarray (8×15K, Arraystar USA).

Techniques: Real-time Polymerase Chain Reaction, Microarray

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: CircRNAs in BALF exosomes and plasma as diagnostic biomarkers in patients with acute respiratory distress syndrome caused by severe pneumonia

doi: 10.3389/fcimb.2023.1194495

Figure Lengend Snippet: Cluster analysis and RT-qPCR validation of differentially expressed circRNAs in the BALF exosomes from ARDS patients and healthy subjects. (A) The cluster analysis plot of differentially expressed circRNAs; red represents upregulated circRNAs, and green represents downregulated circRNAs. (B) The scatter plot shows differences in circRNA expression in BALF exosomes from case–control groups. The x- and y-axes in the figure represent the mean normalized signal values (log2 scaled) of the sample group. The green fold line represents± 2|-fold, and therefore, the icon above or below the green line represents more than -fold upregulation or downregulation. (C) Volcano plot visualization shows differentially expressed circRNAs. Vertical bars show fold change >| ± 2| (log2 fold change); horizontal green lines represent p = 0.05, −log10 ( p -value), and red dots in the figure represent circRNAs with significant expression differences in statistics. (D) RT-qPCR validation of the nine circRNAs with the most significant differences reveals that seven circRNAs with significant differences between groups are consistent with the gene microarray screening results, with four circRNAs significantly upregulated and three significantly downregulated in the case group. BALF, bronchoalveolar lavage fluid; ARDS, acute respiratory distress syndrome.

Article Snippet: Hybridization was then performed with Arraystar Human circular RNA microarray (13,617, 8 × 15K), and data extraction was carried out using the Agilent Scanner G2505C (Jamul, CA, USA).

Techniques: Quantitative RT-PCR, Biomarker Discovery, Expressing, Control, Microarray